Atherosclerosis is the most common type of vascular disease; it starts from the lesion of intima. Generally, there are lipid and complex carbohydrate accumulation, hemorrhage and thrombosis, fibrous tissue hyperplasia and calcinosis, and gradual metamorphosis and calcification of the middle layer of the artery. The lesion often involves large and medium muscular arteries, once developed enough to block the arterial lumen, the tissue or organ supplied by the artery will be ischemic or necrotic. Atherosclerosis is the most common and important disease in atherosclerotic vascular disease. It is the main pathological basis of ischemic cardiovascular and cerebrovascular diseases such as coronary atherosclerotic heart disease (CHD) and cerebrovascular disease. Among them, cardiovascular disease is the main cause of death worldwide, and the most influential is atherosclerotic cardiovascular disease.

Angiogenesis is the formation of new blood vessels due to the development of existing capillaries and postcapillary veins. The first vessels in the developing embryo form through vasculogenesis, after which angiogenesis is responsible for most, if not all, blood vessel growth during development and in disease.

In normal adult coronary arteries, endothelial and vascular smooth muscle cells are nondividing tissue. However, during growth and development and under conditions of ischaemia, hypoxia, inflammation or other stresses, these cells start to migrate and divide. Blood vessel formation passes through several steps, including: the dissolution of the bond between the endothelium and the underlying basement membrane, migration, adhesion and reattachment of the endothelial cells, proliferation and tube formation. Cellular and molecular changes are required for this process, which is complex and as yet poorly understood.

Magic™ hybridoma growth factor is a special supplement which can successfully increase the cloning efficiency and raise the survival rate of hybridoma cell when added in the hybridoma culturing medium with no negative effect to hybridoma cells. It is a perfect replacement of feeding cells in the production of rat and mouse monoclonal antibodies, for example, fusion, subcloning and cell screening.